Ethyl Methanesulfonate (EMS) Mutagen Toxicity-Induced DNA Damage, Cytosine Methylation Alteration, and iPBS-Retrotransposon Polymorphisms in Wheat (Triticum aestivum L.)

The use of mutagens in plant breeding is used to create new germplasm, increase agricultural yield, quality, and resistance diseases pests. Mutagens are physical or chemical factors that can alter the DNA RNA structure an organism, causing mutations above expected level. One most common potent EMS (ethyl-methane sulfonate), which produces point plants, but a lesser degree also cause loss deletion chromosomal region. This study inter-primer binding site (iPBS) coupled restriction enzyme digestion (CRED-iPBS) technique analysis determine effect on methylation rates wheat genotypes at seedling growth stage. Treatments with five different concentrations (0%; control, 0.1%, 0.2%, 0.3%, 0.4%) four times (0; 3, 6, 9 h) were used. Inter-primer markers employed assess genomic instability cytosine treated wheat. In seeds times, disappearance regular bands formation due effects mutagen revealed genetic diversity exists. CRED-iPBS 3 h + 0.1% treatment produced highest MspI polymorphism value (19.60%), while lowest (10.90%). mutagenic treatments had considerable variety impacts According current research, mutagenesis may be practical method for accelerating programs produce enough populations. Mutation-assisted subsequent selection desirable mutants using carried out utilizing integrated strategy.